Background
Genetic transformation is a process which entails taking a gene from one organism, and inserting it into another so as to alter or add a trait. Uses of genetic transformation include modifying plants to resist frost or pests, strengthening agriculture; altering bacteria to help in environmental disasters by giving them the capability to digest oil; and gene therapy, in which sick cells are transformed with the use of healthy genes.
To move genes from one organism to the other, the gene must be isolated and added to a plasmid. Plasmids are a small circular pice of DNA in addition to the chromosome. They allow bacteria to share genes and help them adapt to their environment more rapidly. To force the bacteria to accept the DNA, the plasmids and bacteria are subjected to heat shock, or a rapid raising and lowering of temperature. This triggers the bacteria's' instincts to absorb new DNA, a survival skill.
This lab involves taking the Green Fluorescent Protein gene from jellyfish and inserting it into bacteria, which will cause them to become bioluminescent.
This lab involves taking the Green Fluorescent Protein gene from jellyfish and inserting it into bacteria, which will cause them to become bioluminescent.
A+ good work!
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